Zebrafish microscopy imaging
Automated microscopy for zebrafish and other whole-organisms screening assay
ACQUIFER is your partner for zebrafish research and imaging. We have a well-established experience in microscopy imaging of zebrafish larvae and other small model-organisms, covering the full experimental workflow from sample mounting, complex imaging to image-analysis.
The ACQUIFER Imaging Machine is the ideal platform for high content screening with such animal models.
It provides highly-stable imaging conditions for time-lapse imaging of non-adherent and motion-sensitive specimen, thanks to its unique opto-mechanical design featuring moving-optics and a static stage.
Besides, the integrated environment control assures optimal in-vivo conditions for image-based screening or long-term observation of multiple developing specimen.
Our latest innovation, the photomanipulation-module streamlines the parallel conduction of laser-ablation, photo-activation or fluorescence lifetime imaging experiments.
Finally, in combination with the ACQUIFER PlateViewer and the script-based Smart Imaging interface, the Imaging Machine enables challenging whole-organism screening workflows.
![](https://www.acquifer.de/wp-content/uploads/2023/10/PV-animation_1000ms_better-quality.gif)
Shown is an animation of a typical zebrafish workflow for organ-specific screening, and workflow control and visualization in the PlateViewer. A pre-screen at low resolution (2x objective) is followed by automated region of interested detection, re-centering of target regions and multi-dimensional image acquisition at higher magnification (10x). Images by Eleonora Lupi.
Example Applications and Assays
![](https://www.acquifer.de/wp-content/uploads/2020/05/CystsKidneyFish-1024x716.png)
Organ-specific screening in cystic kidney zebrafish disease models. See also: Pandey et al. (2019).
![](https://www.acquifer.de/wp-content/uploads/2020/05/kidneyTox-1024x683.png)
High content screening in zebrafish for developmental nephrotoxicity of approved drugs. Click image to watch animation. See also: Westhoff et al (2020).
![](https://www.acquifer.de/wp-content/uploads/2020/05/Montage-1024x683.jpg)
Screening for chemical modulators of heart development in zebrafish. Images by 4DHeart ESR Eleonora Lupi (Nadia Mercader lab, University of Bern & Acquifer).
![](https://www.acquifer.de/wp-content/uploads/2020/05/heartrate.gif)
Heartbeat quantification in medaka and zebrafish embryos under physiological conditions. See also Gierten et al. (2020).
![](https://www.acquifer.de/wp-content/uploads/2020/05/kidneyFunction-1024x359.png)
Assessment of renal function in larval zebrafish. See also Steenbergen et al. (2020).
![](https://www.acquifer.de/wp-content/uploads/2020/05/cancerFish-1024x723.png)
Embryonic zebrafish xenograft assay of cancer metastasis – Image courtesy of Arwin Groenewoud (Ewa Snaar-Jagalska lab, Leiden University).
![](https://www.acquifer.de/wp-content/uploads/2020/05/virus.gif)
Disease modelling of viral infections. Image courtesy of ImageInLife ESR Valerio Laghi (Jean-Pierre Levraud lab, Institut Pasteur, Paris).
![](https://www.acquifer.de/wp-content/uploads/2020/05/bacterialBurden-1024x730.png)
Bacterial burden assay in zebrafish. Image courtesy of ImageInLife ESR Salomé Muñoz Sánchez (Annemarie Meijer Lab, Leiden University).
![](https://www.acquifer.de/wp-content/uploads/2020/05/medaka-movie-preview-1024x692.png)
Time-lapse imaging to characterize different anesthesia conditions in medaka. Click image to watch movie. See also Lischik et al. (2019).
![](https://www.acquifer.de/wp-content/uploads/2020/05/tail.gif)
Tail cut assays to study inflammation in zebrafish. Image courtesy of Yufei Xie, Mahmoud Halima (Annemarie Meijer and Marcel Schaaf labs, Leiden University).
![](https://www.acquifer.de/wp-content/uploads/2023/03/neutrophils.gif)
Monitoring of neutrophil migration after automated laser injury. Image data generated by INFLANET ESRs Nils Olijhoek (Stephen Renshaw and Phil Elks labs, University of Sheffield) and Sankeert Satheesan (ACQUIFER)
![](https://www.acquifer.de/wp-content/uploads/2020/05/segmentationZebrafish.gif)
Imaging oscillating reporter gene expression to study pattern formation in zebrafish segmentation. Image courtesy of Daniele Soroldoni and Andy Oates lab, EPFL.