Imaging machine
for High Content Screening

  • Smart Image Acquisition
  • Designed for experiment reproducibility
  • Unique optical and mechanical design for sensitive specimen
  • Data storage & processing integrated

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Why IMaging Machine?

We developed our Imaging Machine for easy, precise, robust and smart high content screening applications without the need for an expert to manage the experiment. We focused on integrated data management, long term data transparency, sensitive samples and a robust machine technology that allows Imaging Machines to learn and reproduce protocols from other devices. In other words: Imaging Machines are designed for everyone to use.

Applications

Zebrafish

  • Dorsal orientation tool
  • Lateral orientation tool
  • Image optimization – Deconvolution
  • Smart Imaging interface (TCP based)
  • Visualization tools

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  • Drosophila
  • Large / thick 3D specimen
  • Image optimization / de-blurring
  • Smart Imaging interface (TCP based)

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(Time-lapse) Cell based

  • Optimized Temperature control – unique laminar flow design for MT plates
  • Light power sensor at sample level
  • precise re-positioning (linear motor axis + 1nm resolution encoder)
  • Laser Autofocus
  • Smart Imaging Interface (TCP based)

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YEAST

  • Static MT plate holder (for very force sensitive yeast lines)
  • Laser Autofocus
  • Yeast optimized image based auto-focus
  • Image processing workflows
  • Robotic sample preparation expertise
  • Smart Imaging interface (TCP based)

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Large projects

  • Designed for single and multi Imaging Machine projects
  • HIVE Data module integration allows seamless project scaling
  • Light power, temperature, motor sensors designed for optimized data reproducibility
  • Small benchtop design optimal for multi machine setups
  • Smart Imaging interface (TCP based)

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Robotic integration

  • TCP/IP interface allows remote control from many software packages
  • Robotic lid allows easy gripper access
  • Sensors for position, light and temperature render hardware calibration routines obsolete

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Technical details

Mechanical designStatic sample holder, moving optics block
Linear Motors with absolute position encoder, 1nm resolution
XY axis range120 mm x 80 mm, 1nm resolution, linear motor, absolute encoded
Z axis range25 mm, 1nm resolution, linear motor , absolute encoded
Z axis designoptimized for speed and precision (for data deconvolution)
Objectives2x, 4x, 10x, 20x, 40x (Nikon)
LED light sourceOmicron LED-HUB , up to 6 modules parallel, temperature controlled
LED light wavelengths385, 405, 455, 470, 505, 528, 595, 625 nm
LED lifetime> 15.000hours on-time
LED power sensorPower Sensor at MT plate level
Fluo Filter blockscustom type, up to 5
Fluo Filter sets monoany 25mm “Semrock” combination – see Semrock-Fluo-table
Fluo Filter sets duo-quadin combination with LED light source module for ultra fast switching
CameraHamamatsu sCMOS 2k x 2k
Temperature + CO2
Incubation typerobotic lid + integrated laminar flow, pressure free
Incubation range20°C – 40°C * if T > ambient temp
CO2via external controller
FocussingLaser Autofocus @780nm
Image based Autofocus (standard, yeast)
Remote controloptional via TCP based commands from any software
Ambient conditions18°-25°C / 65° – 77 ° F (Indoor use)
Relative humidity 30%-80% (without condensation)
Power120v-240V
Weight62kg
H x W x D553mm x 528mm x 555mm

Dimensions

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Your Imaging Machine Support

Phone: +49 (6221) 435 2034 E-Mail: support@acquifer.de