Imaging machine
for High Content Screening

  • Smart Image Acquisition
  • Designed for experiment reproducibility
  • Unique optical and mechanical design for sensitive specimen
  • Data storage & processing integrated

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Why IMaging Machine?

We developed our Imaging Machine for easy, precise, robust and smart high content screening applications without the need for an expert to manage the experiment. We focused on integrated data management, long term data transparency, sensitive samples and a robust machine technology that allows Imaging Machines to learn and reproduce protocols from other devices. In other words: Imaging Machines are designed for everyone to use.

Applications

Zebrafish

  • Dorsal orientation tool
  • Lateral orientation tool
  • Image optimization – Deconvolution
  • Smart Imaging interface (TCP based)
  • Visualization tools

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  • Drosophila
  • Large / thick 3D specimen
  • Image optimization / de-blurring
  • Smart Imaging interface (TCP based)

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(Time-lapse) Cell based

  • Optimized Temperature control – unique laminar flow design for MT plates
  • Light power sensor at sample level
  • precise re-positioning (linear motor axis + 1nm resolution encoder)
  • Laser Autofocus
  • Smart Imaging Interface (TCP based)

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YEAST

  • Static MT plate holder (for very force sensitive yeast lines)
  • Laser Autofocus
  • Yeast optimized image based auto-focus
  • Image processing workflows
  • Robotic sample preparation expertise
  • Smart Imaging interface (TCP based)

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Large projects

  • Designed for single and multi Imaging Machine projects
  • HIVE Data module integration allows seamless project scaling
  • Light power, temperature, motor sensors designed for optimized data reproducibility
  • Small benchtop design optimal for multi machine setups
  • Smart Imaging interface (TCP based)

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Robotic integration

  • TCP/IP interface allows remote control from many software packages
  • Robotic lid allows easy gripper access
  • Sensors for position, light and temperature render hardware calibration routines obsolete

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Technical details

User interface “App based” or “Standard” or “Advanced” or “Smart imaging” or “Remote Master”
Specimen specific apps Zebrafish, yeast, drosophila, cell. organoid, spheroid
Mechanical design static sample holder, moving optics block
Linear Motors with absolute position encoder, 1nm resolution
XY axis range 120 mm x 80 mm, 1nm resolution, linear motor, absolute encoded
Z axis range 25 mm, 1nm resolution, linear motor , absolute encoded
Z axis design optimized for speed and precision (for data deconvolution)
Objectives 2x, 4x, 10x, 20x, 40x (Nikon)
LED light source Omicron LED-HUB , up to 6 modules parallel, temperature controlled
LED light wavelengths 340, 369, 385, 405, 455, 470, 505, 528, 595, 625, 660, 730, 820 nm
LED lifetime > 15.000hours on-time
LED power sensor Power Sensor at MT plate level
Fluo Filter blocks custom type, up to 5
Fluo Filter sets mono any 25mm “Semrock” combination – see Semrock-Fluo-table
Fluo Filter sets duo-quad in combination with LED light source module for ultra fast switching
Camera Hamamatsu sCMOS 2k x 2k
Temperature + CO2
Incubation type robotic lid + integrated laminar flow, pressure free
Incubation range 20°C – 40°C * if T > ambient temp
CO2 via external controller
Focussing Laser Autofocus @780nm
Image based Autofocus (standard, yeast)
Remote control optional via TCP based commands from any software
Ambient conditions 18°-25°C / 65° – 77 ° F (Indoor use)
Relative humidity 30%-80% (without condensation)
Power 120v-240V
Weight 62kg
H x W x D 553mm x 528mm x 555mm

Dimensions

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Your Imaging Machine Support

Phone: +49 (6221) 435 2034 E-Mail: support@acquifer.de