Imaging machine
for High Content Screening

  • Smart Image Acquisition
  • Designed for experiment reproducibility
  • Unique optical and mechanical design for sensitive specimen
  • Data storage & processing integrated

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Why IMaging Machine?

We developed our Imaging Machine for easy, precise, robust and smart high content screening applications without the need for an expert to manage the experiment. We focused on integrated data management, long term data transparency, sensitive samples and a robust machine technology that allows Imaging Machines to learn and reproduce protocols from other devices. In other words: Imaging Machines are designed for everyone to use.

Applications

Zebrafish

  • Dorsal orientation tool
  • Lateral orientation tool
  • Image optimization – Deconvolution
  • Smart Imaging interface (TCP based)
  • Visualization tools

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  • Drosophila
  • Large / thick 3D specimen
  • Image optimization / de-blurring
  • Smart Imaging interface (TCP based)

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(Time-lapse) Cell based

  • Optimized Temperature control – unique laminar flow design for MT plates
  • Light power sensor at sample level
  • precise re-positioning (linear motor axis + 1nm resolution encoder)
  • Laser Autofocus
  • Smart Imaging Interface (TCP based)

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YEAST

  • Static MT plate holder (for very force sensitive yeast lines)
  • Laser Autofocus
  • Yeast optimized image based auto-focus
  • Image processing workflows
  • Robotic sample preparation expertise
  • Smart Imaging interface (TCP based)

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Large projects

  • Designed for single and multi Imaging Machine projects
  • HIVE Data module integration allows seamless project scaling
  • Light power, temperature, motor sensors designed for optimized data reproducibility
  • Small benchtop design optimal for multi machine setups
  • Smart Imaging interface (TCP based)

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Robotic integration

  • TCP/IP interface allows remote control from many software packages
  • Robotic lid allows easy gripper access
  • Sensors for position, light and temperature render hardware calibration routines obsolete

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Technical details

Mechanical designStatic sample holder, moving optics block
Linear Motors with absolute position encoder, 1nm resolution
XY axis range120 mm x 80 mm, 1nm resolution, linear motor, absolute encoded
Z axis range25 mm, 1nm resolution, linear motor , absolute encoded
Z axis designoptimized for speed and precision (for data deconvolution)
Objectives2x, 4x, 10x, 20x, 40x (Nikon)
LED light sourceOmicron LED-HUB , up to 6 modules parallel, temperature controlled
LED light wavelengths385, 405, 455, 470, 505, 528, 595, 625 nm
LED lifetime> 15.000hours on-time
LED power sensorPower Sensor at MT plate level
Fluo Filter blockscustom type, up to 5
Fluo Filter sets monoany 25mm “Semrock” combination – see Semrock-Fluo-table
Fluo Filter sets duo-quadin combination with LED light source module for ultra fast switching
CameraHamamatsu sCMOS 2k x 2k
Temperature + CO2
Incubation typerobotic lid + integrated laminar flow, pressure free
Incubation range20°C – 40°C * if T > ambient temp
CO2via external controller
FocussingLaser Autofocus @780nm
Image based Autofocus (standard, yeast)
Remote controloptional via TCP based commands from any software
Ambient conditions18°-25°C / 65° – 77 ° F (Indoor use)
Relative humidity 30%-80% (without condensation)
Power120v-240V
Weight62kg
H x W x D553mm x 528mm x 555mm

Dimensions

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Application Notes

The ACQUIFER Imaging Machine: An automated microscopy platform for high- throughput screening of budding yeast

The budding yeast Saccharomyces cerevisiae, an eukaryotic microorganism, is a powerful model organism to address biomedical research questions on the genome-scale using growth or biochemical assays. This is complemented by large-scale imaging screens using automated high-throughput microscopy to visualize fluorescent reporter localizations. This allows monitoring the full yeast proteome via GFP fusion proteins or any phenotype that can be followed by a fluorescent marker. Due to the small cell sizes, photosensitivity and non- adherence of yeast cells, these high throughput screening assays demand advanced automated imaging platforms that are capable of robustly and reproducibly acquiring high-resolution datasets for visualization and scoring of cellular and sub-cellular phenotypes.
Download application note

ACQUIFER Smart Imaging: An interface for remote controlling and unsupervised feedback microscopy on the Imaging Machine

High content screening is routinely employed to automatically acquire multi-dimensional image datasets at fixed positions within wells of microtiter plates. While this is sufficient for many applications, it imposes rather inflexible screening workflows as imaging positions are pre-defined by users, often regardless of specimen location or sample characteristics. This can lead to acquisition of unnecessary datasets, omission of features of interest and could hinder more complex assays that would depend on real-time analysis of image data.
Download application note

The ACQUIFER PlateViewer: A tool for visualizing high content screening data and supervised feedback microscopy

Modern High Content Screening microscopes allow rapid automated imaging of entire microtiter plates by imaging fixed positions within each well. This is ideal for in-vitro cell culture based readouts or other assays with evenly distributed phenotypes. However, it imposes limitations when large specimen or rare events are studied. The limited field of view of objectives often force users to utilize low magnification lenses leading to low resolution data or accept the omission of features of interest in many wells.
Download application note

Brochures

Download Imaging Machine brochure

Your Imaging Machine Support

Phone: +49 (6221) 435 2034 E-Mail: support@acquifer.de